
Does Pre-Workout Increase Testosterone?
Many gym-goers wonder whether pre-workout supplements can influence testosterone levels. Pre-workout supplements boost energy and performance, but their effect on testosterone is unclear.
Ingredients like caffeine, creatine, and zinc may help, yet results vary. Lifestyle factors such as training, sleep, and diet have a much stronger impact
Before exploring further, please read the disclaimer located at the end of this webpage.
Key Takeaways
- Pre-workouts are formulated to enhance energy, focus, and endurance, not specifically to increase testosterone levels.
- Limited evidence supports a link between pre-workouts and testosterone boost, and effects vary by individual and ingredients.
- Lifestyle habits like strength training, proper nutrition, quality sleep, and stress control have a greater influence on testosterone.
A Simple Explanation of Pre-Workout
Pre-workout supplements are products used before exercise, usually in powder form or ready-to-drink beverages. Their main purpose is to boost energy, reduce fatigue, and help users maintain endurance.
Formulations vary between products but typically include carbohydrates for quick energy, caffeine for increased alertness, amino acids to support muscles, and other compounds that may enhance exercise performance.
Animal studies suggest potential benefits, but these findings have limitations, and human clinical trials are needed to confirm their effects.
Does Pre-Workout Increase Testosterone?

Whether pre-workout increases testosterone remains uncertain and depends on individual response and nutrition. Some ingredients, such as caffeine, creatine, beta-alanine, and BCAAs, may support healthy testosterone levels, but it is best to check testosterone before and after use to evaluate effectiveness.
Ingredients That May Support Testosterone

Caffeine
Caffeine is a common ingredient in many pre-workout supplements, stimulating the central nervous system to increase alertness and reduce fatigue.
Some studies suggest that caffeine may indirectly promote temporary increases in testosterone during high-intensity exercise by enhancing performance and reducing oxidative stress.
For example, a 2024 analysis suggested that caffeine can stimulate endogenous testosterone production, supporting strength and endurance (Gür et al., 2024). However, these effects are inconsistent and depend on dosage and individual response.
Beta-alanine
Beta-alanine is an amino acid commonly included in pre-workout formulas, helping increase carnosine levels in muscles, which can enhance endurance and reduce fatigue. When used alone, beta-alanine does not appear to significantly affect testosterone [2].
However, a 2022 study suggested that combining beta-alanine with creatine may help promote testosterone and improve exercise performance [3]. The effect of beta-alanine on this hormone still requires more evidence, and human clinical trials are needed to confirm these findings.
Creatine
Creatine is one of the most studied ingredients in sports supplementation, known for its ability to increase strength and muscle mass. It may also support natural testosterone production, especially when combined with high-intensity resistance training [4].
Therefore, creatine is often considered an effective and safe choice for enhancing performance and supporting hormone health, although more human clinical trials are needed to confirm its effects on testosterone.
Theanine
Unlike ingredients aimed directly at increasing strength, theanine stands out for its relaxing and stress-reducing properties. This naturally occurring amino acid, abundant in green tea leaves, helps calm the nervous system and improve sleep quality.
While it does not directly increase testosterone, theanine may indirectly support hormonal balance by reducing stress and enhancing sleep, two factors important for overall hormone health (Nobre, 2022).
BCAAs
BCAAs are a group of essential amino acids, including leucine, isoleucine, and valine, commonly used to aid muscle recovery after exercise.
Some studies suggest that BCAA supplementation may help the body maintain testosterone levels better during intense training, while reducing stress hormones and supporting faster recovery (Sharp & Pearson, 2010).
As a result, BCAAs are considered a useful option for individuals engaged in high-intensity workouts.
Zinc and magnesium
Zinc and magnesium are two essential minerals that play a key role in maintaining male hormonal health. Zinc is directly involved in testosterone production, so a deficiency often leads to a significant decrease in this hormone (Te et al., 2022).
Meanwhile, magnesium not only supports metabolic activities but has also been shown to increase both total and free testosterone, particularly in individuals who exercise or train regularly [8].
The combination of these two minerals contributes significantly to maintaining hormonal balance and improving male physiological function.
Pre-Workout Ingredients to Avoid for Safety

When choosing a pre-workout, users should be careful to avoid ingredients that may be harmful to health. Some notable examples include:
DMAA: This is a compound structurally similar to amphetamine. Using DMAA can have serious cardiovascular effects, increasing blood pressure and the risk of stroke. For this reason, the FDA has banned its use in dietary supplements.
BMPEA: This is a stimulant with a high potential risk because it may raise blood pressure and is associated with stroke risk. Since its safety has not been established, BMPEA should not be present in pre-workout products.
Proprietary blends: These are “secret” or “exclusive” formulas where manufacturers do not clearly disclose the amount of each ingredient. This can result in less effective products or unsafe dosages for users.
Other Factors to Consider
Beyond the ingredients in a pre-workout, its effects on testosterone also depend on several other factors. Important aspects to consider include:
Individual variability: Each person has different natural testosterone levels, so responses to pre-workout supplements will vary and cannot be generalized.
Dosage and formulation: The amount and combination of ingredients in the product may determine whether a pre-workout has a significant effect on testosterone.
Overall health and lifestyle: Factors such as diet, exercise habits, and sleep quality play a crucial role, sometimes having a stronger impact than pre-workout supplementation itself.
Safe Tips Before Using Pre-Workout for Testosterone
Before using any pre-workout to support testosterone, you should consider several important points to ensure effectiveness and minimize health risks. The following tips can help you choose and use products more safely:
Check the ingredients: Before using any pre-workout product, carefully read the ingredient list to understand what you are putting into your body. This helps avoid excessive intake of certain substances and ensures the product suits your needs and current health status.
Talk to a doctor: If you have underlying health conditions or are taking medications, consulting a healthcare professional is essential to prevent interactions or unwanted effects.
Check for third-party seals: Prefer pre-workouts tested by reputable organizations such as NSF Certified for Sport or Informed-Sport to ensure quality and transparency.
Start small: When trying a pre-workout for the first time, begin with a small dose to monitor your body’s response. This approach helps reduce the risk of side effects such as digestive issues or jitteriness.
Frequently Asked Questions
1. Can Pre-Workouts Damage Testosterone?
There is no clear evidence that pre-workouts directly lower testosterone. On the other hand, many common ingredients in pre-workouts, such as creatine, have been shown to support increases in testosterone and growth hormone during high-intensity training [9].
2. Which Exercise Will 100% Increase Testosterone?
No exercise can guarantee a 100% increase in testosterone, as hormone levels also depend on diet, sleep, and overall health. However, research shows that resistance training, especially compound movements engaging multiple muscle groups, can temporarily raise testosterone [10]. Additionally, HIIT has been observed to positively affect free testosterone levels [11].
3. Does Pre-Workout Affect Sperm?
Pre-workout may affect sperm if it contains ingredients that disrupt hormones or impair male reproductive function. However, when free from potentially harmful compounds and used within safe limits, there is currently no evidence that pre-workouts directly harm sperm.
Conclusion
In summary, the current body of research does not provide strong evidence that pre-workout supplements directly increase testosterone.
While certain ingredients may temporarily influence energy, focus, or exercise performance, their effects on hormone levels remain inconsistent and highly individual.
Testosterone is far more influenced by overall lifestyle factors such as resistance training, sleep quality, balanced nutrition, and stress management. Therefore, pre-workout should be viewed as a performance aid rather than a guaranteed hormone booster.
Testimonial Disclaimer
*The testimonials presented on this website are provided by individuals based on their personal experiences with our products. These testimonials represent individual opinions and experiences, which may not be typical or applicable to all users of our products. Results may vary depending on a variety of factors, including individual health, lifestyle, and adherence to product usage instructions.References
- [1] Gür, M., Çınar, V., Akbulut, T., Bozbay, K., Yücedal, P., Aslan, M., Avcu, G., Padulo, J., Russo, L., Rog, J., & Migliaccio, G. M. (2023). Determining the Levels of Cortisol, Testosterone, Lactic Acid and Anaerobic Performance in Athletes Using Various Forms of Coffee. Nutrients, 16(19), 3228. https://doi.org/10.3390/nu16193228
- [2] Hoffman J, Ratamess NA, Ross R, Kang J, Magrelli J, Neese K, Faigenbaum AD, Wise JA. Beta-alanine and the hormonal response to exercise. Int J Sports Med. 2008 Dec;29(12):952-8. doi: 10.1055/s-2008-1038678. PMID: 18548362.
- [3] Samadi M, Askarian A, Shirvani H, Shamsoddini A, Shakibaee A, Forbes SC, Kaviani M. Effects of Four Weeks of Beta-Alanine Supplementation Combined with One Week of Creatine Loading on Physical and Cognitive Performance in Military Personnel. Int J Environ Res Public Health. 2022 Jun 29;19(13):7992. doi: 10.3390/ijerph19137992. PMID: 35805647; PMCID: PMC9265371.
- [4] Hoffman J, Ratamess N, Kang J, Mangine G, Faigenbaum A, Stout J. Effect of creatine and beta-alanine supplementation on performance and endocrine responses in strength/power athletes. Int J Sport Nutr Exerc Metab. 2006 Aug;16(4):430-46. doi: 10.1123/ijsnem.16.4.430. PMID: 17136944.
- [5] Nobre AC, Rao A, Owen GN. L-theanine, a natural constituent in tea, and its effect on mental state. Asia Pac J Clin Nutr. 2008;17 Suppl 1:167-8. PMID: 18296328.
- [6] Sharp CP, Pearson DR. Amino acid supplements and recovery from high-intensity resistance training. J Strength Cond Res. 2010 Apr;24(4):1125-30. doi: 10.1519/JSC.0b013e3181c7c655. PMID: 20300014.
- [7] Te L, Liu J, Ma J, Wang S. Correlation between serum zinc and testosterone: A systematic review. J Trace Elem Med Biol. 2023 Mar;76:127124. doi: 10.1016/j.jtemb.2022.127124. Epub 2022 Dec 23. PMID: 36577241.
- [8] Cinar V, Polat Y, Baltaci AK, Mogulkoc R. Effects of magnesium supplementation on testosterone levels of athletes and sedentary subjects at rest and after exhaustion. Biol Trace Elem Res. 2011 Apr;140(1):18-23. doi: 10.1007/s12011-010-8676-3. Epub 2010 Mar 30. PMID: 20352370.
- [9] Lane, M. T., & Byrd, M. T. (2018). Effects of Pre-Workout Supplements on Power Maintenance in Lower Body and Upper Body Tasks. Journal of Functional Morphology and Kinesiology, 3(1), 11. https://doi.org/10.3390/jfmk3010011
- [10] Jansson, D., Lindberg, A., Lundberg, E., Domellöf, M., & Theos, A. (2022). Effects of Resistance and Endurance Training Alone or Combined on Hormonal Adaptations and Cytokines in Healthy Children and Adolescents: A Systematic Review and Meta-analysis. Sports Medicine - Open, 8(1), 1-20. https://doi.org/10.1186/s40798-022-00471-6
- [11] Herbert, P., Hayes, L., Sculthorpe, N., & Grace, F. (2017). HIIT produces increases in muscle power and free testosterone in male masters athletes. Endocrine Connections, 6(7), 430. https://doi.org/10.1530/EC-17-0159
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